Estrogen modulates transactivations of SXR-mediated liver X receptor response element and CAR-mediated phenobarbital response element in HepG2 cells
نویسندگان
چکیده
منابع مشابه
Thyroid hormone can increase estrogen-mediated transcription from a consensus estrogen response element in neuroblastoma cells.
Thyroid hormones (T) and estrogens (E) are nuclear receptor ligands with at least two molecular mechanisms of action: (i) relatively slow genomic effects, such as the regulation of transcription by cognate T receptors (TR) and E receptors (ER); and (ii) relatively rapid nongenomic effects, such as kinase activation and calcium release initiated at the membrane by putative membrane receptors. Ge...
متن کاملHPV E6 Knockdown Restores Adenovirus Mediated-Estrogen Response Element Linked p53 Gene Transfer in HeLa Cells
p53 is inactivated and degraded by the E6 protein encoded by the integrated human papillomavirus (HPV) in the cervical cancers (Scheffner et al., 1990). The susceptibility of p53 to degradation by the E6 protein varies between the arginine and proline variants at codon 72 of p53 (Storey et al., 1998). In vivo studies demonstrate that the arginine p53 variant is seven times more susceptible to d...
متن کاملEstrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter.
We have shown previously that estrogen-stimulated transcription from the human lactoferrin gene in RL95-2 endometrium carcinoma cells is mediated through an imperfect estrogen response element (ERE) at the 5 -flanking region of the gene. Upstream from the ERE, a DNA sequence (-418 to -378, FP1) was selectively protected from DNase I digestion by nuclear extracts from endometrial and mammary gla...
متن کاملpS2 Gene expression in HepG2 cells: complex regulation through crosstalk between the estrogen receptor alpha, an estrogen-responsive element, and the activator protein 1 response element.
The pS2 promoter is complex with binding sites for a number of protein factors that may participate in modulating its activity. The pS2 gene was transcriptionally activated by estrogens in HepG2 cells transformed (HepER3) to express the estrogen receptor alpha (ERalpha). The phorbol ester phorbol 12-myristate 13-acetate (PMA) stimulated pS2 expression in both HepER3 and the parental, non-ER-exp...
متن کاملassessing the levels of camp response element binding protein1 (creb1) after sirna mediated knockdown in k562 cells
objective: creb1 is an important downstream protein for many signaling pathways. by designing efficient sirnas against creb1, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. in this research the efficiency of creb1 knockdown by two different sirnas in k562 cells has been studied. materials and methods: sirnas have been designed accordi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Experimental and Molecular Medicine
سال: 2010
ISSN: 1226-3613,2092-6413
DOI: 10.3858/emm.2010.42.11.074